No, not a good idea to run a preamp without all of the tubes in place even for a moment. They present loads within the circuit. You wouldn't run without a resistor or cap, would you? Damage can result. Any tech should be able to look under the bonnet and tell you in a split second what's what or, if you have confidence, why not just sub in your new high end tubes. If you sub one in and you can't hear a difference then either it's not in the line stage or, if it is it doesn't matter anyway 'cause you can't hear the difference.