I disconnected the pre and had just the speakers cables to the speakers
Kolledog
If I understand you correctly you had the the offending Amp completely isolated from the rest of the audio system. Just the Amp alone with nothing connected to the line input. Only the speaker was connected, and you still had the hum problem.
Sounds to me the problem is within the Amp its self. When you moved the Amp you may have caused and internal problem. Maybe nothing more than a tube just happened to go bad.

