I would say change out all 6. Compared to the new production (affordable) Russian and Chinese tubes, the old tubes and Shuguang tubes sound more 3D and less glarey. The stock tubes should sound very nice, but specialized tubes should adda lot of depth to the soundstage, and a more natural timbral balance. I didn't find any one tube position that didn't affect the sound, be it gain stage, CCS, or driver. You could argue that some affect it more than others, but it's hard to tell. If I had to say, I'd say the CCS tube has the least impact, but it does have noticeable impact. Or I'm delusional.